Analysis of HMW-GS in Pedigree of Longdong Dryland Winter Wheat Varieties

作者:Xiao; YANG; Zicheng; LIU; Guanghong; JIA College; of; Agriculture; and; Forestry; Gansu; Dryland; Research; Center; of; Winter; Wheat; Germplasm; Innovation; and; Application; Engineering; Longdong; University; Qingyang; 745000; China

摘要:The composition of high molecular weight glutenin subunits(HMW-GS)was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryland winter wheat germplasm resources had N,7+8,2+12 as the dominant HMW-GS composition(42.86%),while the dominant HMW-GS composition in the introduced germplasms was N,7+9,2+12(36.84%).On the Glu-B1 locus of Longdong dryland germplasms,subunit 7+8 had the highest frequency,accounting for 65.71%,and on the Glu-B1 locus of the introduced germplasms,subunit 7+9 had the highest frequency,accounting for 57.89%.Whether subunit 7+8 is related to drought resistance in varieties still needs further study.On Glu-D1 locus,high-quality subunit 5+10 appeared in the introduced resources for 5 times,accounting for 13.16%of all the introduced lines,and appeared in Longdong dryland winter wheat germplasm resources for 2 times,only accounting for 2.86%of all the resources,suggesting that Longdong dryland germplasms lack high-quality subunits and need further improvement.

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